An in vitro Comparative study upon the Hemolytic, Thrombogenic, Coagulation parameters and Stability properties of the Hemiscorpius lepturus Venom

r, Seyedian and s.m, Hoseiny and s, Kamyab and r, Mansury and n, Seyedian and s, Gharibi and a, Zare Mirakabadi (2014) An in vitro Comparative study upon the Hemolytic, Thrombogenic, Coagulation parameters and Stability properties of the Hemiscorpius lepturus Venom. Archives of Razi Institute, 69 (1).

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Abstract

Hemiscorpius lepturus belonging to Hemiscorpiidae family is the most venomous of all types of scorpion existing in south west of Iran causing hemoglobinuria and dermal lesions by envenomation. We compare the hemolytic pattern upon time in different domestic animals upon time according to their different sphingomyelin contents. In addition other in vitro hematologic parameters, platelet lysis, coagulation changes and finally preservative factors (temperature, pH, protases) are discussed. The hemolytic activity was inhibited significantly by heating at 100 °C for 60 minutes (26%) and reached 38% via incubation with papain (10U/ml) while retained over a pH range of 4-11. Horses and sheep have the lower (61%) and upper (100%) rate of hemolysis. Calcium and magnesium ions could increase rate of hemolysis and EDTA solution had significantly decresing effect. The venom significantly changed in vitro coagulation factors (PT and APTT) from base line levels and had no effect on platelet lysis. It seems that our venom belongs to metalloproteinases due to potentiation effects of bivalent cations (calcium and magnesium) and ghost cell formation in our study indicatiing hemoglobin efflux.

Item Type: Article
Additional Information: indexer :farshid ravansalar
Uncontrolled Keywords: Hemiscorpius lepturus, Stability Hemolysis, Ghost cell, Metalloenzyme
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Dentistry
Depositing User: Unnamed user with username karvarz1
Date Deposited: 19 Dec 2015 18:40
Last Modified: 19 Dec 2015 18:40
URI: http://eprints.bpums.ac.ir/id/eprint/4343

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