A novelmethodforextractionofaproteinous coagulant from Plantago ovata seeds forwater treatmentpurposes

Ramavandi, Bahman and Hashemi, Seyedenayat and Kafaei, Raheleh (2015) A novelmethodforextractionofaproteinous coagulant from Plantago ovata seeds forwater treatmentpurposes. MethodsX, 2. pp. 278-282.

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Abstract

Severalchemicalshavebeenappliedintheprocessofcoagulantextractionfromherbalseeds,andthebest extractionhasbeenobtainedinthepresenceofKClorNaNO3 [1–3], andNaCl [4]. However,themainchallenge posed tothesemethodsofcoagulantextractionistheirrelativelylowefficiency forwatertreatmentpurposesand the formationofdissolvedorganicmatterduringthetreatmentprocess.Inthesemethodsthesalts,whichhavea one-valancemetal(Na+ and K+), aredepositedintheinternalstructureandtheporeofthecoagulant,andmaybe useful forthecoagulation/flocculation process.Inthisresearch,wefoundthatmodified methodsproducedmore dense protein.Therefore,themodified procedurewasbetterthantheolderoneforremovalofturbidityand harness fromthecontaminatedwater.Herewedescribeamethodwhere: � AccordingtotheHardy–Schulze rule,weappliedtheFe3+ ions insteadofNa+ and K+ for theextractionofprotein from Plantago ovata seeds. � The methodwasnarrowedtoextractproteinbyethanol(defatting)andammoniumacetateandCM-Sepharose (proteinextraction). � Two consecutiveelutriationsofcrudeextractwasdirectlyperformedusing0.025-MFeCl3 and 0.05-MFeCl3 according tothebasisoftheion-exchangeprocesses.

Item Type: Article
Additional Information: indexer:anis najafi
Uncontrolled Keywords: Plantagoovata seeds, Protein,Extraction,Coagulant
Divisions: Faculty of Medicin
Depositing User: Unnamed user with username karvarz2
Date Deposited: 17 Jul 2016 03:11
Last Modified: 17 Jul 2016 03:11
URI: http://eprints.bpums.ac.ir/id/eprint/4466

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