Effect of Genistein and L-Carnitine and Their Combination on Gene Expression of Hepatocyte HMG-COA Reductase and LDL Receptor in Experimental Nephrotic Syndrome

YOUSEFINEJAD, Abbas and SIASSI, Fereydoon and MIRSHAFIEY, Abbas and ESHRAGHIAN, Mohammad-Reza and KOOHDANI, Fariba and JAVANBAKHT, Mohammad Hassan and SEDAGHAT, Reza and RAMEZANI, Atena and ZAREI, Mahnaz and DJALALI, Mahmoud Effect of Genistein and L-Carnitine and Their Combination on Gene Expression of Hepatocyte HMG-COA Reductase and LDL Receptor in Experimental Nephrotic Syndrome. Iran J Public Health, 44 (10). pp. 1339-1347.

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Abstract

Background: Nephrotic syndrome is a disorder that leads to hyperlipidemia. L-carnitine and genistein can effect on lipid metabolism and the syndrome. In the present study, we have delved into the separate and the twin-effects of L-carnitine and genistein on the gene expressions of HMG-COA reductase and LDL receptor in experimental nephrotic syndrome. Methods: In this controlled experimental study, 50 male Sprague–Dawley rats were randomly divided into five groups: NC (normal-control), PC (patient-control), LC (L-carnitine), G (genistein), LCG (L-carnitine-genistein). Adri-amycin was used for inducing nephrotic syndrome and the spot urine samples and urine protein-to-creatinine ratio were measured. Hepatocytic RNA was extracted and real-time PCR was used for HMG-COA Reductase and LDL receptor gene Expression measurement. Results: The final weight of the patients groups were lower than the NC group (P=0.001), and weight gain of the NC group was higher than the other groups (P<0.001). The proteinuria and urine protein-to-creatinine ratio showed sig-nificant differences between PC group and LC, G and LCG groups at week 7 (P<0.001). The expression of HMG-COA Reductase mRNA down regulated in LC, G and LCG groups in comparison with PC group (P<0.001). ΔCT of LDLr mRNA showed significant differences between the PC group and the other patient groups (P<0.001). Conclusion: This study shows a significant decreasing (P<0.001) and non-significant increasing trend in HMG-COA Reductase and LDLr gene expression, respectively, and synergistic effect of L-carnitine and genistein on these genes in experimental nephrotic syndrome.

Item Type: Article
Subjects: QU Biochemistry
WY Nursing
WZ History of Medicine
Divisions: Faculty of Medicin > Department of Biochemistry
Depositing User: سپیده مقدسی
Date Deposited: 03 Jul 2018 08:16
Last Modified: 03 Jul 2018 08:16
URI: http://eprints.bpums.ac.ir/id/eprint/6215

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