Seyedian, R and Fard, E.S and Najafiasl, M and Assadi, M and Zaeri, S (2020) N-acetylcysteine-loaded electrospun mats improve wound healing in mice and human fibroblast proliferation in vitro: A potential application of nanotechnology in wound care. Iranian Journal of Basic Medical Sciences, 23 (12). pp. 1590-1602. ISSN 20083866
|
Text
IJBMS_Volume 23_Issue 12_Pages 1590-1602.pdf Download (1MB) | Preview |
Abstract
Objective(s): N-acetylcysteine (NAC) has gained attention recently in dermatology as a unique antioxidant. In light of progress in nanotechnological methods, it was hypothesized that loading NAC onto nanofibers would positively affect skin wound healing. The objective of this study was to fabricate NAC-loaded electrospun mats and test their effect on wound healing in vivo and in vitro. Materials and Methods: Polyvinyl alcohol (PVA)-based mats loaded with NAC at three concentrations were electrospun and characterized in terms of physicochemical properties and drug release profile. Human fibroblast cells (in vitro) and mouse full-thickness skin wounds (in vivo) were treated with mats for 5 and 14 days, respectively. Wound area, tissue histopathology, fibroblast proliferation and cellular oxidative state were evaluated. Results: Mats containing 5% PVA/NAC showed thinner fibers with suitable physicochemical properties and a sustained drug release profile. PVA/NAC (5%) mats enhanced fibroblast proliferation and attachment in vitro. The mats resulted in significant wound closure with high levels of re-epithelialization and collagen fiber synthesis on day 14 post-surgery in vivo. The mats also reduced granulation tissue and edematous stroma to a higher extent. These findings were accompanied by a significant decrease in tissue lipid peroxidation and higher superoxide dismutase activity, which may explain how NAC improved wound healing. Conclusion: We propose an NAC-loaded nanofibrous mat that takes the advantage of a porous nanoscaffold structure to release NAC in a sustained manner. This mat may be a promising candidate for further clinical evaluation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Electrospun nanofiberIn vitroMouse N-acetylcysteine (NAC)Oxidative stressWound |
| Subjects: | QV pharmacology |
| Divisions: | Faculty of Medicin > Department of Pharmacology |
| Depositing User: | خدیجه شبانکاره |
| Date Deposited: | 20 Dec 2020 08:58 |
| Last Modified: | 20 Dec 2020 08:58 |
| URI: | http://eprints.bpums.ac.ir/id/eprint/8979 |
Actions (login required)
 |
View Item |
