Obeidi, N and Tamaddon, G and Ranjbaran, R and Khamisipour, G and Saberi, F (2020) Expression analysis of gamma globin gene switching associated micro RNAs, flowing BCL11A gene inhibition. Gene Reports, 21. ISSN 24520144
Full text not available from this repository.Abstract
Background/aim: MicroRNAs are small, single-stranded RNA molecules that are targeting between 10% and 30% of the human genome, also a gene that binds to several miRNAs. By connecting them to targets gene, the silencing occurs after the translation. Materials and methods: The aim of this study was to investigate changes in the expression of miRNAs before and after inhibiting the gamma-beta gene switch by siRNA against the BCL11A gene. MiR- 15a, miR- 16, miR-486-3p, miR- 23a and miR-27a, important miRs in switching gamma to beta gene, were investigated. Peripheral blood mononuclear cells were isolated by Ficoll-Paque. They were cultured in the IMDM medium containing supplementary materials for differentiation to erythroid series for a period of 28 days and stored in a 37-degree incubator containing 5% CO2. Every 2–3 days, identification of the cells was done. On the 21st day, cells were introduced by insertion of siRNA against BCL11A. Results: The expression of miR- 15a in the cultured cells gradually increased from the first day to the eighteenth day and decreased on 21 day. Expression pattern of miR- 16 was increased on the 8th day and decreased on the 22th day. MiR-23a decreased on day 8, increased on day 21, and remained unchanged on days 18 and 22. miR-27a increased the expression on day 8, decreased on day 21, and remained unchanged on days 18 and 22. Expression of miR-486-3p increased on the 8th, 18th and 21th days; also, on the 22thday, there was a decrease in expression of miR-486-3p. Conclusion: Our data showed that changes in the expression of miRNAs were significant. The results of this study suggest that inhibition of the gamma gene switch to beta, keeps hemoglobin F high, therefore, this could lead to improved symptoms in patients with hemoglobinopathy.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BCL11A Gamma globin Hemoglobinopathy miRNAs siRNA |
| Subjects: | QW Microbiology and Immunology |
| Divisions: | Faculty of Paramedics |
| Depositing User: | خدیجه شبانکاره |
| Date Deposited: | 20 Dec 2020 09:33 |
| Last Modified: | 20 Dec 2020 09:33 |
| URI: | http://eprints.bpums.ac.ir/id/eprint/8989 |
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