Comparison of various methods for DNA extraction from human isolated paraffin-embedded hydatid cyst samples

Barazesh, A and Fouladvand, M and Rayani, M and Ebrahimi, S (2020) Comparison of various methods for DNA extraction from human isolated paraffin-embedded hydatid cyst samples. Journal of Parasitic Diseases, 44 (3). pp. 613-617. ISSN 09717196

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Official URL: https://link.springer.com/article/10.1007/s12639-0...

Abstract

Successful molecular research with reliable results depends on achieving significant and uniform amounts of genomic DNA from the parasite as the first and most basic step. Therefore, selection of an appropriate method that minimizes damage to the DNA of the parasite, is very important. In this study, we are going to describe a method that can extract DNA from human isolated paraffin-embedded hydatid cysts with a high quality and quantity. Formalin fixed and Paraffin-embedded hydatid cyst samples isolated from human lung and archived in the pathology laboratory were used for this purpose. Several sections of the paraffin blocks were prepared with 5 micron thickness and DNA were extracted by three different methods including; modified boiling, commercial kit and the method described by Larissa A. Pikor et al. The obtained DNA were evaluated by Nanodrop in terms of the yield of DNA and possible contaminations. To compare the quality of DNA prepared, cox1 region was amplified using specific primers. It was found that the DNA extracted by modified boiling had the lowest rate of contamination and the best electrophoretic band on the gel, compared to other two performed methods. Considering the findings of this study, this simple and high throughput DNA extraction method with high yield and quality can be recommended for extraction of DNA from formalin fixed and paraffin-embedded hydatid cysts.

Item Type: Article
Uncontrolled Keywords: (DNA extraction) (Hydatid cysts) (Paraffin-embedded)
Subjects: QX Parasitology
Divisions: Research Center > Persian Gulf Marine Biotechnology Research Center
Depositing User: خدیجه شبانکاره
Date Deposited: 26 Dec 2020 08:21
Last Modified: 26 Dec 2020 08:21
URI: http://eprints.bpums.ac.ir/id/eprint/9128

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