Rezaei, N and Hamidabadi, H.G and Khosravimelal, S and Zahiri, M and Ahovan, Z.A and Bojnordi, M.N and Eftekhari, B.S. and Hashemi, A and Ganji, F. and Darabi, S and Gholipourmalekabadi, M (2020) Antimicrobial peptides-loaded smart chitosan hydrogel: Release behavior and antibacterial potential against antibiotic resistant clinical isolates. International Journal of Biological Macromolecules, 164. pp. 855-862. ISSN 01418130
Full text not available from this repository.Abstract
In this study, we synthesized thermo-responsive chitosan (TCTS) hydrogels, and loaded with different concentrations of antimicrobial peptide (AMP) (0, 4, 8 and 16 μg·ml−1) to fabricate an antibacterial wound dressing against resistant clinical isolates. Physico-chemical properties, release behavior, cytobiocompatibility and antibacterial activity of the AMP-TCTS hydrogels against standard strain and resistant Acinetobacter baumannii were fully determined in vitro. The TCTS-40% β-glycerolphosphate hydrogels showed a gelation time of 15 min at 37 °C. 80% weight loss at day 35 with no changes in pH value was observed. AMP-TCTS hydrogels showed a burst release of AMP (around 40%) at day 1, and a controlled release up to day 7. A dramatic water uptake was observed at first 4 h, and then continued for 10 h in a steady manner. All the AMP-TCTS hydrogels showed excellent cytobiocompatibility for human fibroblasts. The TCTS showed no antibacterial activity against both standard strain and clinical isolates. All the AMP-TCTS hydrogels had strong antibacterial activity against standard strains, but only 16 μg·ml−1 showed antibacterial behavior against resistant A. baumannii. Our results strongly suggest the 16 μg·ml−1 AMP-TCTS hydrogel as an excellent antibacterial wound dressing against resistant A. baumannii, and now promises to proceed with pre-clinical investigations.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Chitosan Hydrogel Resistant bacteria, post-wound infection Thermos-responsive Wound dressing |
| Subjects: | QS Human Anatomy |
| Divisions: | Faculty of Medicin |
| Depositing User: | خدیجه شبانکاره |
| Date Deposited: | 26 Dec 2020 08:16 |
| Last Modified: | 26 Dec 2020 08:16 |
| URI: | http://eprints.bpums.ac.ir/id/eprint/9140 |
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