Hosseinpour, M and Mashayekhi, K and Falak, R and Jamalzehi, S and Haftcheshmeh, S.M and Mousavi, M.J and Soleimani, A and Koushki, K and Sankian, M and Soukhtanloo, M (2020) Production and Characterization of Monoclonal Antibody against Vit v1: A Grape Allergen Belonging to Lipid Transfer Protein Family. Iranian Journal of Allergy, Asthma and Immunology, 19 (2). pp. 139-148. ISSN 17351502
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Abstract
Allergy to non-specific lipidtransfer protein (nsLTP), the major allergen of grape (Vit v1), is considered as one of the most common fruit allergies in Iran. Therefore, a specific monoclonal antibody (mAb) can be used for the characterization and assessment of. Accordingly, this study aimed to generate and characterize a mAb against Vit v1 with a diagnostic purpose. To this end, Vit v1 allergen (9 kDa) was extracted using a modified Bjorksten extraction method. Natural Vit v1-immunized mouse splenocytes were fused with SP2/0Ag-14 myeloma cells for generating hybridoma cells. Specific antibody-secreting Hybridoma cells were selected using ELISA. Finally, anti-Vit v1 mAb was characterized by western blotting, ELISA, and isotyping methods. In the current study, a 9 kDa (Vit v1) protein was attained fromcrude and fresh juice of grape extracts and the isotype of desired anti-Vit v1 mAb was determined as IgM with K light chain. In addition, The ELISA results demonstrated that anti-Vit v1 mAb was specified against natural Vit v1 in the grape cultivar and related LTP allergens, such as Pla or 3 (p<0.0001). In the present study, a specific mAb was produced for detecting the LTP allergen. This mAb with a confirmed specificity can be utilized for evaluating the LTP allergens and their allergenicity in different grape cultivars. © 2020 Tehran University of Medical Sciences
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Allergy Grape Lipid transfer protein Monoclonal antibody Non-specific lipid transfer protein |
| Subjects: | QU Biochemistry |
| Divisions: | Faculty of Medicin > Department of Biochemistry |
| Depositing User: | خدیجه شبانکاره |
| Date Deposited: | 28 Dec 2020 05:57 |
| Last Modified: | 28 Dec 2020 05:57 |
| URI: | http://eprints.bpums.ac.ir/id/eprint/9177 |
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