Dobaradaran, S and Soleimani, F and Taherkhani, R and Spitz, J and Saeedi, R (2021) Molecular detection of E. coli and Vibrio cholerae in ballast water of commercial ships: a primary study along the Persian Gulf. Journal of Environmental Health Science and Engineering, 19 (1). ISSN 2052336X
Full text not available from this repository.Abstract
Purpose: Ballast water is one of the most important ways for the transfer of aquatic organisms such as Escherichia coli (E. coli) and Vibrio cholerae. The aim of this study was to investigate Mdh gene of E. coli and the OmpW gene of Vibrio cholerae bacteria by PCR technique in the ballast water of commercial ships entering Bushehr port along the Persian Gulf. Methods: In this study, 34 samples of ballast water entered Bushehr port were studied by using culture and PCR methods to determine Mdh gene of E. coli and OmpW gene of Vibrio cholerae. Genomic DNA of bacterial strains was extracted and PCR was performed by using specific primers of E. coli and Vibrio cholerae. Results: The specific Mdh gene of E. coli was detected in 4 ballast water samples and the positive samples were analyzed by antisera methods for E. coli O157:H7. Results of antisera showed that there were 3 positive samples of O157:H7 serotype. The results of the PCR technique showed that the OmpW gene of Vibrio cholerae was negative for all positive culture samples. Conclusions: Further studies are highly recommended to monitor other aquatic organisms in ballast water to protect the marine environment.
| Item Type: | Article |
|---|---|
| Subjects: | T Technology > TD Environmental technology. Sanitary engineering |
| Divisions: | Faculty of Engineering, Science and Mathematics > School of Civil Engineering and the Environment |
| Depositing User: | خدیجه شبانکاره |
| Date Deposited: | 28 Jun 2021 05:53 |
| Last Modified: | 28 Jun 2021 05:53 |
| URI: | http://eprints.bpums.ac.ir/id/eprint/9297 |
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