Khoradmehr, A and Emadi, E and Shams, A and Kalantar, S.M (2021) Appropriate fixative for MEM-G/9 staining of cultured human HLA-G-positive JEG-3 trophoblast tumor cells. Biotechnic and Histochemistry. ISSN 10520295
Full text not available from this repository.Abstract
Human leukocyte antigen (HLA-G) participates in immunosuppression and is useful for prenatal diagnostics. Isolation of fetal cells positive for HLA-G by HLA-G antibody conjugated nanoparticles from the cervix of pregnant women is the basis for non-invasive prenatal testing. Endocervical specimens are fixed in transport medium before isolation using antibody conjugated nanoparticles. Staining of HLA-G using MEM-G/9 antibody, however, is restricted to unfixed cells. We investigated the effect of several fixatives on the interaction of HLA-G with MEM-G/9 in the HLA-G-positive cell line, JEG-3. We investigated absolute methanol, 1:1 acetate buffer:methanol, Pap solution and paraformaldehyde. The effects of these fixatives were evaluated using immunofluorescence. We found no MEM-G/9 surface staining of methanol fixed cells. Approximately 40% of JEG-3 cells fixed with paraformaldehyde failed to stain. Nearly all cells were stained with MEM-G/9 following fixation with acetate buffer:methanol or Pap solution. Our findings indicate the importance of using an appropriate fixative for preserving HLA-G cell surface antigen for studies using the MEM-G/9 antibody.
| Item Type: | Article |
|---|---|
| Subjects: | QU Biochemistry |
| Divisions: | Research Center > Persian Gulf Marine Biotechnology Research Center |
| Depositing User: | خدیجه شبانکاره |
| Date Deposited: | 04 Jul 2021 08:18 |
| Last Modified: | 04 Jul 2021 08:18 |
| URI: | http://eprints.bpums.ac.ir/id/eprint/9370 |
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