Isoenzyme characterization of trichomonas vaginalis isolated from hiv patients in fars and kerman, Southeast Iran

Rayani, M and Vafafar, A and Ziaali, N and Tavakoli, R and Rayani, M (2021) Isoenzyme characterization of trichomonas vaginalis isolated from hiv patients in fars and kerman, Southeast Iran. Journal of Kerman University of Medical Sciences, 28 (2). 179 -186. ISSN 10239510

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Abstract

Background: Trichomonas vaginalis is an anaerobic flagellated protozoan which is responsible for human urogenital infections. Several zymodemes of T. vaginalis have been reported from various parts of the worlds on the basis of isoenzyme patterns. This study was conducted to characterize the isolated organisms of T. vaginalis from HIV patients using isoenzyme electrophoresis in Fars and Kerman provinces, southeast Iran. Methods: Eighteenmass cultivated isolates of T. vaginalis in themodified TYI-S-33medium were analyzed using isoenzyme electrophoresis. Polyacrylamide gel electrophoresis (PAGE) of five different enzyme systems were used to characterize T. vaginalis isolates: (i) Glucose-6-phosphate dehydrogenase (G6PD), (ii) Glucose phosphate isomerase (GPI), (iii) Malate dehydrogenase (MDH), (iv) Malic enzyme (ME), and (v) Phosphoglucomutase (PGM). Results: MDH, GPI, PGM, and ME enzyme systems showed a homogeneity and detected an identical enzyme pattern in all isolates. Meanwhile, G6PD revealed two different enzyme patterns. The isoenzyme electrophoretic profiles divided 18 T. vaginalis isolates into two zymodemes. Zymodeme 1 contained Shiraz isolates and zymodeme 2 contained Kerman isolates. Conclusion: The polymorphism of Iranian human isolates of T. vaginalis could be assessed by biochemical study using appropriate enzyme systems. Isoenzyme analysis is a promising method for the characterizationof T. vaginalis. New molecular studies with increased number of enzyme loci and genetic markers are suggested to classify more zymodemes of Trichomonas in Iran.

Item Type:	Article
Subjects:	WC Communicable Diseases
Divisions:	Research Center > The Persian Gulf Tropical Medicine Research Center
Depositing User:	خدیجه شبانکاره
Date Deposited:	04 Sep 2021 06:51
Last Modified:	04 Sep 2021 06:51
URI:	http://eprints.bpums.ac.ir/id/eprint/9404

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