Matrigel enhances differentiation of human adipose tissue-derived stem cells into dopaminergic neuron

Absalan, F and Pasandi, M.S and Ghasemi Hamidabadi, H and Saeednia, S and Bojnordi, M.N and Zahiri, M and Alizadeh, R and Bagher, Z (2021) Matrigel enhances differentiation of human adipose tissue-derived stem cells into dopaminergic neuron. Neuroscience Letters, 760. ISSN 03043940

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Background: Therapy based stem cells have offered a novel therapeutic approach for the improvement of neurodegenerative diseases, specially Parkinson. Hence, developing a well-established culture model with appropriate stem cells is extremely crucial in regenerative engineering to provide efficient targeted cells. Human adult mesenchymal stem cells derived from adipose tissue (hADSCs) have emerged as a promising source of stem cells due to their unique potentials of self-renewal and differentiation into other stem cells. The purpose of this study was to investigate the differentiation capacity of hADSCs into dopaminergic and neuron-like cells in the 3D culture plate (Matrigel). Methods and materials: hADSCs were obtained from adipose tissues of patients and then characterized morphologically with flowcytometry. Isolated cells were harvested to perform differentiation on Matrigel and tissue culture plate (TCP) supplemented with induction factors. The survival rate of cells during neural induction was monitored by MTT. The expression of specific cell markers was analyzed by QRT-PCR and immunocytochemistry on days 2, 8 and 14. The level of released dopamine was measured using HPLC technique. Results: Matrigel had a positive effect on maintaining cell growth compared to those on TCP. Moreover, the number of TH and MAPII positive cells is substantially higher in Matrigel than in TCP. Sox2 and Nestin had a prominent expression in hADSCs within the first days of differentiation. The gene expression of neural markers such as TH, Nurr1, LMX1A and DAT was detected and increased after day 8. Moreover, the dopamine released in the cell harvested on Matrigel was greater than those seeded on TCP. Conclusions: Overall, hADSCs could generate dopaminergic cells, which suggest its strong capability to serve as a tool for Parkinson disease model in the regenerative medicine.

Item Type: Article
Uncontrolled Keywords: Human adipose tissue-derived stem cellsDifferentiation3-DimensionalDopaminergic neuron
Subjects: QT Physiology
Divisions: Faculty of Medicin > Department of Anatomy
Depositing User: خدیجه شبانکاره
Date Deposited: 27 Dec 2021 08:44
Last Modified: 27 Dec 2021 09:51

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